High Ssensitivity Domoic Acid(ASP) ELISA Test Kit

Domoic Acid(ASP) ELISA Test Kit

CH 98011

Product Description

      The Domoic Acid ELISA is an immunoassay for the quantitative and sensitive detection of Domoic Acid. Domoic Acid is one of the toxins associated with amnesiac shellfish poisoning (ASP). This test is suitable for the quantitative and/or qualitative detection of Domoic Acid in water samples as well as shellfish samples. For shellfish samples a sample preparation is required.

Sample Preparation(Mussels and other shellfish)

1. Mussels are removed from their shells, washed with deionized water, thoroughly dried and homogenized (Polytron or equivalent). 2. A 0.5 g portion of the homogenized mussels is then mixed with 2 mL Methanol/deionized water (50/50) (Polytron or equivalent). Vortex ~1 min. 3. Centrifuge mixture for 15 minutes at 4000 g. Collect the supernatant. 4. Remove 20 µL and dilute to 1.0 mL with Sample Dilution Buffer (1:50). 5. Analyze diluted extracts as samples (see Assay Procedure). The Domoic Acid concentration contained in the samples is determined by multiplying the concentration of the diluted extract by a factor of 200 to account for extraction (step 2) and dilution (step 4). Highly contaminated samples outside the range of the curve should be diluted further and re-analyzed. Samples with low concentrations of Domoic Acid or samples which must meet specific regulatory levels may be analyzed at lesser dilutions.