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商品の詳細:
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| 急速な: | <30分 | 高度 回復: | 80-105％ |
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| 検出時間: | 1hours | 保管温度: | 2-8°C |
| 仕様: | 96t | 線形間隔: | 0.2ng/ml |
Okadaic Acid(DSP) ELISA Test Kit
CH 98012
The Okadaic Acid ELISA is an immunoassay for the quantitative and sensitive detection of Okadaic Acid. Okadaic Acid is one of the toxins associated with diarrheic shellfish poisoning (DSP). This test is suitable for the quantitative and/or qualitative detection of Okadaic Acid in water samples as well as shellfish samples.
The test is a direct competitive ELISA based on the recognition of Okadaic Acid by specific antibodies. Okadaic Acid, when present in a sample and an okadaic acid-enzyme-conjugate compete for the binding sites of rabbit anti-okadaic acid antibodies in solution. The okadaic acid antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Okadaic Acid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
| Kit Contents | Amount | Storage |
| Second antibody-Coated Plate | 1 x 96-well Plate(8 wells x 12 strips) | 2-8°C |
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Okadaic Acid(DSP) Standards: Negative control 0.2 ng/mL 0.5 ng/mL 1 ng/mL 2ng/mL 5ng/mL
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1 mL
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-20°C |
| Antibody Solution | 8 ml | 2-8°C |
| Okadaic Acid-HRP Conjugate | 15 mL | |
| 5X Wash Solution | 50mL | |
| Stop Buffer | 7mL | |
| Color Solution | 7 mL | |
| Sample Diluent Concentrate (10X) | 17 mL |
Okadaic Acid ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored
1. Mussels are removed from their shells, washed with deionized water, thoroughly dried and homogenized using a Polytron or equivalent.
2. A 1.0 g portion of the homogenized mussels is then mixed with 6 mL methanol/deionized water (80/20) using aPolytron or equivalent.
3. Centrifuge mixture for 10 minutes at 3000 x g. Collect the supernatant.
4. Add 2 mL methanol/deionized water (80/20) to the mussel tissue residue. Re-centrifuge mixture for 10 minutes. Collect supernatant and add to the first portion.
5. Bring final volume of collected supernatant to 10 mL with methanol/deionized water (80/20). Filter the extract through a 0.45µm syringe filter (Millex HV, Millipore or equivalent).
6. Add 990 µL of 1X Sample Diluent Buffer to a 4 mL glass vial with lined cap. Add 10 µL of the filtered extract to the diluent (1:100 dilution). Vortex.
7. Analyze diluted extracts as samples.
The Okadaic Acid concentration contained in the samples is determined by multiplying the concentration of the diluted extract by a factor of 1000. Highly contaminated samples outside the range of the curve should be diluted further and re- analyzed. Samples with low concentrations of Okadaic Acid or samples which must meet specific regulatory levels may be analyzed at lesser dilutions.
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